Skip to main content

Pepar Chromatography & Radixl Pepar Chromatography


> Pepar  Chromatography 

Www.industrialchemistry.com


- Papar Choromatography is an 
analytical methad used to separate colour chemical or subdance 

- Paper chromatography is usefull for the separating complox mixture of compound having similar properly for e.g amino acid.
- The molecule phase is a solution that travell up the stationany phase due to capillany action
- The moleculer phase is generally an  alcohol Solvent mixed up. whild the stationany Phase a strip of chromatography paper
- cellulose filter paper is oftean used as the stationadiy phase in papar chromatography
- Since it is hydu1ophilic it is usually covered with a then field of water.

>  Porinciple
- This techiques is a type of partition chromatography in which the substance are distributed betweern two liguid conce is Stationary liquid usually (wateor)which is held in filter of the paper & called the Stationory Phase, the other is the moving liquid or developing solvent & called the moving phase.
- The componetnt of the mixture to be seperated migrate at different rate & paper as spots at diffectent on the paper.
- Originally pepar chromatography was used to seperated mixture of organic substances such dyes & amino acid only .
- in this lachnics a drop of the stest so  ion is applied as a small spot or a filter the Spols is dry.
- The paper is kept in a close chember  & the edge, of the paper is dipped in to solvent called developing solvent.
- As soon as the filter Paper gets the liquid through it's capillary axis & when rich the spot of the test solution the varion substances are move by solvent systm at various speed.
- when the solvent as move to a suiteble hight (15-18cm) the paper dried & varios spots are visuallize by suitable greant visuallizing agent.
- The moment of substances relative is the solvent expressed in the terms of Rf value (migration parameter).

> Migration Perameter
- The position of migrated spots on the
chromatography are indicated by different term such as Rf.Rx

> Types Of PC

1) Dessending paper chromatography 
- in  this type development of the chromatography in chromatoram is done by allowing the solvent to down the paper

2) Ascending paper chromatography
-  Here the solvent  travell upward direction of the chromatography paper both the descending &  ascending paper chromatography are used for separation of organic & inorganic substances.
3) Agending-Dessending paper chromatography
- It is hyteride of both the above technique the upper part of the ascending Chromatogram can be folded over a roll and allowing the paper to and becomes desceding after crossinig the roll.

> Redixl Pepar Chromatography

Www.industrialchemistry.com

- it is  also colled a circular chromatogrsphy 

- here a circular fillter pepar is take  & the Sample is giver at the conters of the pepar solvent
- So that wick of the paper is dip inside the solvent
- The solvent raises through the wick & the compound gel sepelaration in form of concentoated circular zone .

Comments

Post a Comment

Popular posts from this blog

Types of rabbur and information about it.

 Rubber is a name of a matenrial which come Strate & Shrinkage. - Rubber is a polymer either peroduced by a natural Source Can be synthesis on an industusial scale. - Rubbeut is also known as elastomer is a high Polymeulr having elastic propenties. - Elasticity & rabberness is the most important Properties of rubber. - on strching it elongate to nearly 200% of its original dimentational &  quickely reaction to it's original Shape when Strching fouice is a remove. >   Importance of application of ruubber  properties of rubber(natural & synthetic rubber) 1)  Grood tensile Strength. 2) High flexibility 3) good electrical resistance 4) good chemical resistance 5) good Abrasion resistance 6) good resilience 7) high elasticity 8) low condudivity 9) low permeability to air, grases & Liquids. - Rubber are use manufacture of large no. of puroducts such as, tyres, v-belts, conveyor belts rubber lined tanks, m...
5 comments
Read more

M.sc Sem-2 Manufacturing Of Glass & Types Of Glass And Information About It

                   >  Process - Glass are made by heating mixtune that nearly always consist of silica call & dry powder or metallic oxides. - Most glass are manufactun by a process in which raw-mateutials are converted at high temperature to a homogejeaus melts , that is then form in to an article. -  Raw mateutials are selecting acrourding the properties purity supply, cost etc . - Silica sand is most common in goradiant all most type of glasses.  - limp-Stones is a sourvr of colcium soda ash is Source of Sadium and bore and Boutic acid is Source of borone. - Broken gloss Usually termed as collected is used as a batch material to enhance glass melting and to resuce the amount of dust and other particular matter that aften a companies a batch mads exculsively from fresh raw-mateuial. - Tipically 10 to 151% of glass batch is the comprised of collect. - More efficient of operation as used in the c...
3 comments
Read more

Industrial Production of MgSo4,CaSo4, Mg(OH)² & MgCo³ & Na²Sio³

1)  Industrial production of MgSo4,CaSo4, Mg(OH)² > Reaction                                     1)  Mgcl² + Ca(OH)²  ----->  Mg(OH)² +                                                            CaCl² 2)  Cacl² + H²So4  ------>  CaSo4 + 2HCL 3)  MgCl² + H²So4 ------> MgSo4 + 2HCL > Process  - In the industrial production of MgSo4 & CaSo4 first of all mixed salt bittern is first taken in a reactor. - Ca( OH)² from line treatment tank is also charge into the reactor. - Due to reactor precipitation of the Mg(OH)² is obtained. - filter the mass in filter press to remove precipitation of Mg(OH)² which is dry pulverised & packed. - Filtrate is further taken in a trea...
4 comments
Read more